Fig. 2.

hnRNP A1 is up-regulated during differentiation of W12E cells. Cell lysates were prepared from undifferentiated (U) and differentiated (D) W12E and HaCaT cells. 10 μg of each extract was fractionated on SDS-PAGE and Western blotted with antibodies against the proteins indicated beside the graphs associated with each set of Western blots. (A) SF2/ASF (B) hnRNP A1 (C) U2AF⁶⁵ (D) Sm protein (E) U1A (F) CstF-64 (G) HuR. Graphs show quantification of the Western blot data by densitometry scanning and show the mean and standard deviation from the mean of three to five separate experiments. The scale on the Y-axis in (A) and (B) is different to the scale for (C–G). (H) Western blots of undifferentiated and differentiated W12 and HaCaT cells probed with antibodies against involucrin, keratin 10 and filaggrin to demonstrate extent of differentiation of the cells. W12 cell extracts were probed with an anti-L1 antibody to demonstrate production of virus late proteins, associated with terminally differentiated, virus-infected epithelial cells. Blots were reprobed with an antibody against GAPDH as a loading control.