Table 1.
Genes on hypoxia-signalling pathway PCR arrays whose expression is up- or down-regulated in human adipocytes by hypoxia
| Gene | Protein | Change with hypoxia (fold) | P value |
|---|---|---|---|
| Upregulated: 24 h | |||
| MT3 | Metallothionein-3 | 614.6 | <0.001 |
| LEP | Leptin | 20.9 | <0.001 |
| VEGF | Vascular endothelial growth factor | 20.7 | <0.001 |
| SLC2A1 | GLUT-1 (facilitative glucose transporter 1) | 12.4 | <0.001 |
| ANGPTL4 | Angiopoietin-like protein 4 | 10.5 | <0.001 |
| BHLHB2 | Basic helix-loop-helix domain containing, class B,2 | 3.94 | <0.001 |
| CYGB | Cytoglobin | 3.64 | <0.001 |
| IL6 | Interleukin-6 | 2.90 | <0.01 |
| IGF2 | Insulin-like growth factor 2 | 2.67 | <0.02 |
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 2.67 | <0.001 |
| GPI | Glucose phosphate isomerase | 2.22 | <0.001 |
| HIF3A | Hypoxia inducible factor 3, α-subunit | 2.01 | <0.001 |
| Downregulated: 24 h | |||
| CASP1 | Caspase 1, apoptosis-related cysteine protease | −4.44 | <0.001 |
| HIF1A | Hypoxia inducible factor 1, α-subunit | −4.11 | <0.001 |
| EPAS1 | Endothelial PAS domain protein 1 | −3.17 | <0.002 |
| SSSCA1 | Sjogren’s syndrome/sclerodoma autoantigen 1 | −3.14 | <0.02 |
| GPX1 | Glutathione peroxidase 1 | −3.07 | <0.05 |
| AGPAT2 | 1-Acylglycerol-3-phosphate O-acyltransferase 2 | −2.55 | <0.002 |
| CAT | Catalase | −2.53 | <0.02 |
| UCP2 | Uncoupling protein 2 | −2.40 | <0.001 |
| PSMB3 | Proteasome subunit, beta type, 3 | −2.15 | <0.002 |
| Upregulated: 4 h | |||
| MT3 | Metallothionein-3 | 121.0 | <0.05 |
| SLC2A1 | GLUT-1 (facilitative glucose transporter 1) | 4.03 | <0.002 |
| VEGF | Vascular endothelial growth factor | 3.72 | <0.001 |
| RPL28 | Ribosomal protein L28 | 3.20 | <0.05 |
| ADM | Adrenomedullin | 3.12 | <0.05 |
| BHLHB2 | Basic helix-loop-helix domain containing, class B,2 | 2.45 | <0.02 |
| LEP | Leptin | 2.19 | <0.05 |
| HK2 | Hexokinase 2 | 2.01 | <0.05 |
Human adipocytes differentiated from preadipocytes in culture were incubated under normoxia (21% O2) or hypoxia (1% O2) for 4 or 24 h. Hypoxia-signalling pathway PCR arrays (Superarray) were used to screen those genes whose expression is hypoxia-sensitive. The genes listed are those where there was a statistically significant (P < 0.05) change of at least 2-fold. The fold changes are derived from 4 groups of normoxic and hypoxic adipocytes at 24 h and 3 groups at 4 h.