Skip to main content
. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Eur Biophys J. 2008 May 27;37(8):1335–1349. doi: 10.1007/s00249-008-0343-7

Figure 7.

Figure 7

(a) The mean error in the position of the origin determined as a function of the number of photons incident on the CCD. The theoretical data was obtained using a computational model for photon and instrument noise, and the mean was calculated from a sequence of 100 different profiles for the PSF (background: ∼430 photons per pixel, effective pixel size: 160nm, quantum efficiency: 93%, σr: 160 electrons, grid size: 7 × 7 pixels). (b) The average value of r2 for the least-squares fit as a function of the number of photons.