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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: J Neurochem. 2008 Sep 16;107(2):533–543. doi: 10.1111/j.1471-4159.2008.05648.x

Fig. 2. Chemopreventive inducers of endogenous Nrf2 induce Srxn1 expression in neurons and glia.

Fig. 2

A) Neuronal cultures were treated with D3T (25 μM), tBHQ (10 μM, Aldrich) or sulforaphane (5 μM, Merck Biosciences) for 4 h followed by RNA extraction and q-RT-PCR analysis of Srxn1 and Sesn2 (normalized to GAPDH). *p<0.05 (Bonferonni two-tailed T-test, n=3-5). B) Western analysis of Srxn1 protein expression in extracts taken from neuronal cultures treated for 24 h with D3T (25 μM). *p<0.05 (n=6). C) Glial cultures were treated with D3T (25 μM) for 4 h followed by RNA extraction and q-RT-PCR analysis of Srxn1, Sesn2 and Hmox1 (normalized to GAPDH, *p<0.05, two-tailed T-test in this and subsequent experiments unless otherwise stated, n=6).