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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: J Neurochem. 2008 Sep 16;107(2):533–543. doi: 10.1111/j.1471-4159.2008.05648.x

Fig. 3. The Srxn1 promoter contains an ARE which is induced by Nrf2 expression in cortical neurons, as is the endogenous gene.

Fig. 3

A) Schematic showing the putative ARE within mammalian Srxn1 promoters. B) Effect of Nrf2 expression on wild-type and mutant Srxn1-Luc reporters (normalized to Renilla control-see methods). Neurons were transfected with Srxn1 reporter plus pcDNA3.1-Nrf2 or pGlobin control plasmid. NB. In this and all experiments “con” denotes pGlobin control vector. *p<0.05 (2-tailed paired T-test, n=3-5). C) Effect of Nrf2 expression on ARE-Luc activity. *p<0.05 (n=5). D) Effect of transfecting pEF-Nrf2 on endogenous Srxn1 expression. Neurons were transfected with the indicated vectors and after 24 h subjected to immunocytochemical analysis of sulfiredoxin expression. Immunofluorescence performed as described (McKenzie et al. 2006). Scale bar = 40 μm. E) Effect of Nrf2 expression on WT and mutant Srxn1-Luc reporters in glial cells *p<0.05 (n=7). F) Effect of Nrf2 expression on ARE-Luc activity in glial cells. *p<0.05 (n=4). G) Effect of Nrf2 expression on WT and mutant Srxn1-Luc reporters in HEK293cells. *p<0.05 (n=3).