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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: J Neurochem. 2008 Sep 16;107(2):533–543. doi: 10.1111/j.1471-4159.2008.05648.x

Fig. 5. Activity-dependent induction of Srxn1 via the ARE/AP-1 site is primarily mediated by AP-1.

Fig. 5

A) Effect of Keap1 expression on activity-dependent induction of reporters of Srxn1 and Sesn2. Neurons were transfected with the reporter plus TK-driven Renilla control vector. 24 h post-transfection, bursts of action potential firing were induced by treatment of neurons with 50 μM bicuculline, and burst frequency was enhanced by addition of 250 μM 4-amino pyridine (Hardingham et al. 2001). Reporter activity was assayed after the indicated time periods. *p<0.05 Bonferonni two-tailed paired T-test (compared to control, BiC/4-AP-stimulated level, n=6). B) Effect of an interfering mutant of AP-1 (TAM67) on activity-dependent induction of the putative ARE/AP-1 composite site, compared to an upstream AP-1 site. Neurons were transfected with the indicated reporter constructs, plus pTK-RL and either control vector or expression vector for TAM67. 24 h post-transfection neurons were stimulated with BiC/4-AP overnight prior to assay of reporter activity (n=5).