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. Author manuscript; available in PMC: 2009 Feb 3.
Published in final edited form as: Oncogene. 2008 Oct 20;28(2):157–169. doi: 10.1038/onc.2008.380

Figure 1. Silencing of CDC37 depletes kinase client proteins.

Figure 1

Silencing of CDC37 expression using siRNAs O3 and O4 in comparison with inactive siRNAs IC3 and IC4, no transfection (NT) and mock transfection (Mk) conditions. (a) Expression of client proteins ERBB2, CRAF, CDK6, CDK4 and GR in HCT116 human colon cancer cells at 4 and 5 days. (b) Expression of client proteins ERBB2, CRAF, CDK6, CDK4 and survivin in HT29 human colon cancer cells at 5 and 6 days. (c) Expression of ERBB2, CRAF, phosphorylated-AKT, AKT and CDK4 in SkBR3 human breast cancer cells at 5 and 6 days. (d) Expression of HSP90, HSP72, ERBB2 and CDK4 in PC3 human prostate cancer cells at 5 and 6 days. The changes in protein expression in this figure were quantitated by densitometry, as shown in Supplementary Figure S2.