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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Nat Cell Biol. 2008 Nov 9;10(12):1411–1420. doi: 10.1038/ncb1799

Figure 3.

Figure 3

Cdc20 requires both APC/C and Mad2 binding motifs to be degraded in the SAC

HeLa cells were microinjected with plasmids encoding YFP- wild-type or mutant Cdc20 as indicated and the fluorescence level assayed through mitosis in the presence of nocodazole. Data are representative of 3 independent experiments for each mutant. A) IR mutant (R499E) 9 cells B) C-box mutant (D77RYIPHR83 to A77AAIAHA83) 13 cells C) Mad2-binding site R132A, 12 cells.