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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Nat Cell Biol. 2008 Dec 14;11(1):78–84. doi: 10.1038/ncb1815

Figure 2. Basipetal, AUX1-mediated auxin transport is required for auxin-dependent root-hair growth.

Figure 2

(a-c) Root-hair phenotype of (a) wild-type, (b) aux1-22 and (c) aux1-22 plants transformed with AUX1::AUX1-YFP (AUX1::AUX1-YFP(aux1-22)). Scale bars represent 500 μm. (d) Frequency distribution of root-hair lengths in wild-type, aux1-22 and AUX1::AUX1-YFP (aux1-22) plants. (e,f) GUS stained roots of 5-day-old seedlings of (e) wild-type and (f) aux1-22 plants carrying the IAA2::GUS auxin response reporter. Double-headed arrow indicates expected location of differentiation zone. Scale bars represent 500 μm. (g) Average root-hair length of aux1-22 plants expressing HA-AUX1 in a tissue specific manner using a transactivation approach. Root-hair length of F1 individuals (J1701>>HA-AUX1, M0028>>UAS::HA-AUX1 and M0013>>UAS::HA-AUX1) and non-crossed siblings (UAS::HA-AUX1, J1701, M0028 and M0013) are shown. All parental lines are in the aux1-22 background. J1701 drives expression in the stele, M0013 drives expression in the lateral root cap, M0028 drives expression in the lateral root cap and epidermis. Error bars represent 95 % confidence intervals (n = 260 hairs from 26 roots).