Figure 3. Non-hair cells are essential for root-hair development, but an auxin response is not required in non-hair cells.
(a-b) Root-hair phenotype of (a) wild-type and (b) wer/myb23 plants. Scale bars represent 500 μm. (c) Frequency distribution of root-hair lengths in wild-type and wer/myb23 plants. (d-h) Disruption of the auxin response through tissue-specific expression of axr3-1 using a transactivation approach. (d) Root-hair-less phenotype of axr3-1. Root-hair phenotype of (e) J2301>>axr3-1 and (f) J0491>>axr3-1 F1 individuals. Scale bars represent 800 μm. (g and h) Expression pattern of the J2301 and J0491 promoters respectively as demonstrated by GFP expression. GFP fluorescence is shown in green. Propidium iodide stained cell walls are shown in red. J2301 is expressed in non-hair cells, J0491 is expressed throughout the root. Scale bars represent 50 μm.