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Phosphorimage of 32P-labeled 16-mer separated by denaturing PAGE after reaction of duplex DNA with 2PI-C6NC2, 2PI-C3NC3, 2PI-C5NC3, 2PI(OH)-C6NC2, or chlorambucil. Numbered bands identify modified DNA species that were isolated and used in the competition assay of Fig. 4. The data show the formation of interstrand crosslinks (band 1), alkylated single strands (bands 2 and 3), and unmodified DNA.
