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. 2008 Oct 29;9(10):2027–2043. doi: 10.3390/ijms9102027

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© 2008 by MDPI

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — Characterization of crzA disruptants. (a) Colony morphology of crzA disruptants on regeneration plates. (b) Schematic representation of the expected genomic pattern of a Δ crzA mutant after double-crossover recombination. (c) Genomic patterns of parental strains and ΔcrzA mutants revealed by PCR using primers Cz5P and CzP3. M: DNA 1kb ladder. Lane 1, BN9Δku70; lanes 2–5, BN9ΔcrzA; lane 6, RHΔku70; lanes 7–10, RHΔcrzA.