Table 2.

Effect of pH on the sporulation.

Medium	Predicted pH of buffer	Tape slide results
		Presence of perithecia			Presence of ascospores
		Week 4	Week 6	Week 8	Week 4	Week 6	Week 8
Unbuffered PDA	n/a	+	+	+	+	+	+
Tris buffered PDA	7.2	+	+	+	+	+	+
	8.0	+	+	+	+	+	+
	9.0	+	+	+	−	−	−
Citrate-phosphate buffered PDA	3.0	−	NT	NT	−	NT	NT
	4.0	−	+	+	−	−	+
	5.0	−	+	+	−	−	+
	6.0	−	−	+	−	−	−
	7.0	−	+	+	−	−	+
Carbonate-bicarbonate buffered PDA	9.2	−	−	−	−	−	−
	10.0	−	−	−	−	−	−
	10.7	−	−	−	−	−	−
Tris-maleate buffered PDA	5.2	−	−	−	+	+	+
	6.0	+	+	+	+	+	+
	7.0	+	+	+	+	+	+
	8.0	+	+	+	−	+	+
	8.6	+	+	+	+	+	+

^a Tape slides were taken from a single agar plate at four, six or eight weeks post-inoculation. Presence or absence of perithecia and ascospores is indicated with a “+” or “−” respectively. Samples not taken are indicated by “NT”.