Table 4.

Displacement of D and S ligands from DS-MIP and rat hypothalamus by ergot derivatives.

Ergot	Ki (μM)
	D ligand		S ligand
	DS-MIPa)	Rat hypothalamusb)	DS-MIPa)	Rat hypothalamusb)
Terguride	0.14 ± 0.01	0.00003 ± 0.000005	7.79 ± 0.17	0.0013 ± 0.0001
Pergolide	0.20 ± 0.01	0.0007 ± 0.0001	12.78 ± 0.09	0.0060 ± 0.0002
Agroclavine	0.40 ± 0.01	0.0015 ± 0.0002	5.31 ± 0.22	0.0012 ± 0.0001
Ergonovine	69.57 ± 0.51	0.0025 ± 0.0001	14.74 ± 0.29	0.0012 ± 0.0001
Ergocryptine	124.88 ± 1.22	0.0028 ± 0.0002	24.85 ± 0.69	0.0100 ± 0.0004
Ergocristine	141.08 ± 1.47	0.0076 ± 0.0001	19.33 ± 0.60	0.0068 ± 0.0002
Ergocornine	216.60 ± 2.59	0.0104 ± 0.0002	27.25 ± 0.78	0.0200 ± 0.0001

^a). Displacement studies using 25 mg of polymer particulate with ergot concentration ranging from 0.02 to 500 μg/mL (0.03–890.20 μM) and 6.25 μg of D (or S) in a final volume of 5 mL (D = 6.60 μM, S = 5.90 μM) were performed at room temperature (28 ± 1 °C) as described in the Experimental Section. Mean ± SD, n = 3.

^b). Displacement studies using 10 mg of protein with ergot concentration ranging from 0.1 to 500 μg/mL (0.16–890.20 μM) and 0.25 μmole of D (or S) in a final volume of 1 mL (250 μM) were performed at 37 °C as described in the Experimental Section. Mean ± SD, n = 4.