SDS-PAGE analysis of His6-Cel48 purification through a Ni-NTA-agarose column. Expression and purification of His6-Cel48 from E. coli PERM407 was performed as described in Methodology. (Lane 1, molecular weight markers; Lane 2, E. coli PERM407 lysate; Lane 3, flow through; Lane 4, protein fraction eluded with 10 mM imidazol; Lanes 5–8, fractions eluted from the column with 200 mM imidazol; Lane 9, ~100 μg of protein from an E. coli PERM407 lysate were separated on a SDS–12% polyacrylamide gel and transferred to nitrocellulose membranes. The blot was probed with a polyclonal anti-Cel48 rabbit antibody which was diluted 10,000-fold and then processed with an ECL Western-blotting analysis system.)