Figure 4.

Inhibition of prostaglandin formation in LPS/IFN-γ-treated RAW264.7 cells by CuDips. RAW264.7 cells (3.5 × 10⁶ cells/T25 flask) were activated with 500 ng/ml LPS and 10 units/ml IFN-γ in serum-free SMEM for 7 hr. The cell monolayers were washed with PBS at t = 7 hr and then incubated in fresh PBS containing 0–10 μM CuDips for 1 hr. The dimethyl sulfoxide vehicle was kept constant at 1% in all flasks. At t = 8 hr of activation, the PBS was removed and analyzed for prostaglandin content by gas chromatography/negative ion chemical ionization mass spectrometry (54). The activated cells secreted 138 ng PGD₂ and 1.4 ng PGE₂ per 10⁶ cells. These values were taken as 100% conversion, respectively. At all concentrations of CuDips, there was a statistically significant difference in eicosanoid production.