Figure 5.

IC₅₀ of luciferase targeted siRNA delivered by complexation with MPGα into ECV304 cells. 24 h before transfection ECV304-cells were seeded into a 96 well plate (1×10⁴ cells per well). siRNA and MPGα were mixed in Opti-MEM^® I (4.2 μM final concentration of peptide) and incubated for 5 min at room temperature. Cells were overlaid with the complexes for 4 h followed by addition of medium supplemented with 10 % FCS. 24 h after transfection, luciferase activity was measured in a plate reader and cell numbers were normalised with the help of fluorescein diacetate. The IC₅₀-value of 0.8 nM was calculated using the program Grafit.