Table 1.
Summary of the cytotoxicity of the various barley fractions tested and their mycotoxin content by the Vicam method.
| Sample | Cytotoxicity* ppb | FB1 ppb | DON ppb | OTA ppb |
|---|---|---|---|---|
| B4 N fraction | 25,000 | n.d. | 4,800 | − |
| B4 A fraction | >50 | − | − | 4 |
| B7 A fraction | >50 | − | − | 1 |
| B9 A fraction | 50 | − | − | 2 |
| B10 N fraction | 6,250 | n.d. | n.d. | − |
| B10 A fraction | >50 | − | − | 6 |
| B11 N fraction | ||||
| FB1 fraction | 50,000 | 40 | 900 | − |
| B11 A fraction | >50 | − | − | 4 |
| B14 N & A fractions | n.d. | n.d. | n.d. | n.d. |
| B21 N fraction | 20,000 | n.d. | 7,500 | − |
| FB1 fraction | ||||
| B30 | 18,000 | n.d. | 7,500 | − |
| B31 N&A fractions | n.d. | n.d. | n.d. | n.d. |
| MB5 N&A fractions | >50,000 | n.d. | n.d. | − |
| MB24 | ||||
| N fraction | >50,000 | 71 | 6,200 | − |
| FB1 fraction | ||||
| PB4 A fraction | 20 | − | − | 2 |
*
Cytotoxicity figure value at half the cytotoxicity level of the control, i.e. 50% of untreated lymphocytes; ppb = parts per billion (μg/kg) in the original sample. As 200 μL are derived from 25g of original sample and 20 μL are used in a total 200 μL of culture fluid per well the actual concentration experienced by the lymphocytes at the highest concentration will be: ¼,000 of this and the lower levels appropriate dilutions thereof; n.d. = not detected; - = not found in these fractions.
FB1 = Fumonisin B1; DON = Deoxynivalenol; OTA = Ochratoxin A; B = barley grain; MB = malted barley; PB = pearl barley.