Fig. 3.

Coordination of a Mn²⁺ cation in the active center of eLtaS. (A) Coordination bonds between Mn²⁺ in eLtaS and the side chains of residues T300, D475, H476, and E255. (B) Coordination bonds between Ca²⁺ in the P. aeruginosa arylsulfatase and side chains of residues D13, D14, HFG51, D317, and N318. (C) Overlay of A and B. (D) Anomalous difference density map of Mn²⁺ bound to eLtaS. (E) Coordination bonds between Mn²⁺ in B. stearothermophilus PGM and side chains of residues D12, S62, D444, and H445. (F) Overlay of A and E.