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Ionizing radiation induces RPA2 phosphorylation dependent on MEC1. Western blot analysis of RPA2 from TWY397 (MEC1) and DLY285 (mec1-1) cells exposed to increasing doses of ionizing radiation (IR). Samples from the exponentially growing cultures prior to α factor treatment (exp) and from the G1-arrested cultures directly prior to release (α) have been included. Equivalent volumes of original cell culture were analyzed.
