Fig. 3.

SLFavir is sequestered into blood cells and has a dramatically enhanced half-life in vivo. (A) After 60 min, whole blood was isolated from treated mice, separated into components and analyzed for drug content by LC-MS. Results are the average of at least three animals. (B) At time 0, adult male mice were injected i.p. with either compound 4 or 6. Whole blood was removed at the indicated times and the plasma drug levels determined. Total inhibitor level was calculated as the concentration of 5 + 6. All results are the average of at least three independent experiments and the error is standard deviation. (C) Plasma samples were dried and resuspended in DMSO for analysis in the HIV protease assay. Results are shown relative to a control (100% = 10 μM drug extracted immediately from whole blood).