Fig. 1.

L31 mAb specifically recognizes CD8⁺ DCs. Fixed and permeabilized B cell depleted BALB/c splenocytes were analyzed by multicolor flow cytometry (Fig. S1). (A) Expression of Langerin, DEC205, and CD8 in CD11c high gated cells (Fig. S1, gate g). (B) RPMs (Fig. S1, gate j) were evaluated for Langerin, DEC205, MMR, and CD68. (C–E) Langerin and DEC205 expression was analyzed in (C) plasmacytoid DCs (Fig. S1, gate k), (D) monocytes (Fig S1, gate n), and (E) granulocytes (Fig. S1, gate l). One experiment representative of 3 is shown.