. Author manuscript; available in PMC: 2009 Feb 5.

Published in final edited form as: J Med Microbiol. 2003 Aug;52(Pt 8):643–651. doi: 10.1099/jmm.0.05181-0

Table 1. Quantification of hypochlorite production.

Hypochlorite (mM) produced by purified MPO (5 mgml⁻¹) in PBS at pH values of 5·5, 6·5 and 7·5 and in the presence of 1, 10 and 100 mM H₂O₂ at 37 °C. Measurements were made at 0·3, 2·0 and 16 min. Mean values (±se) of three independent experiments. nd, None detected.

H₂O₂ (mM)	Minutes	pH 7·5	pH 6·5	pH 5·5
1	0·3	0·09 ± 0·16	0·35 ± 0·12	0·19 ± 0·11
	2·0	0·02 ± 0·16	0·23 ± 0·12	0·24 ± 0·19
	16·0	nd	0·28 ± 0·12	0·22 ± 0·08
10	0·3	1·14 ± 0·22	2·02 ± 0·79	4·84 ± 0·09
	2·0	0·99 ± 0·10	1·86 ± 0·57	4·44 ± 0·09
	16·0	1·30 ± 0·05	1·57 ± 0·16	3·49 ± 0·09
100	0·3	1·29 ± 0·40	14·80 ± 1·13	13·26 ± 1·58
	2·0	0·18 ± 0·37	8·64 ± 0·59	7·61 ± 1·32
	16·0	0·82 ± 0·26	6·80 ± 0·55	4·16 ± 0·37