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. 2009 Feb 11;4(2):e4436. doi: 10.1371/journal.pone.0004436

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Ferrets were intranasally administered the H5N1 VN04 ca or HK03 ca viruses on day 0. Five and ten days post-inoculation, ferrets were sacrificed to collect paratracheal lymph nodes (TLN) and a B cell ELISpot assay was performed using lymphocytes isolated from TLN and BPL-inactivated H5N1 HK03 ca virus or rH5 HA antigens. The number of IgM ASC (A) and IgG ASC (B) are presented as per 10⁶ lymphocytes.