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Readthrough of the in-frame TGA codon of mouse Sps2 in COS-7 cells and analysis of the contribution of the 3′ UTR to the degree of efficiency of translation. The inverted triangle represents the positioning of the FLAG sequence at the N terminus of the Sps2 cDNA constructs. Anti-FLAG-immunopurified lysates were analyzed by Western blotting using anti-FLAG M2 antibody. Mammalian cells were transfected with the pME18X expression vector without insert as control (VECTOR).
