Skip to main content
. 2009 Feb 13;5(2):e1000374. doi: 10.1371/journal.pgen.1000374

Figure 5. EGFR;PI3K induces glial neoplasia via Pnt and Stg expression.

Figure 5

(A–D,F–H) late 3rd instar larval brain hemispheres. Staining patterns are shown alone (left panels) and with overlaid repo-Gal4-driven CD8GFP (green) and/or Repo (blue) glial markers (right panels). Close-ups of medial anterior regions; anterior up; midline to left. 20 µm scale bars. (A,B): Diphospho-ERK (P-ERK, red) staining in wild-type (A) and repo>dEGFRλ;dp110CAAX (B) brains. High levels of nuclear ERK (arrows) are present in dEGFRλ;dp110CAAX glia, but not in wild-type glia. Glial cell bodies are labeled green in overlay (right panels). 2 µm optical sections. (C,D): PntP1 expression (red) in wild-type (C) and repo>dEGFRλ;dp110CAAX (D) brains. Intense PntP1 expression in dEGFRλ;dp110CAAX glial nuclei (arrows) is coincident with Repo and visible as purple nuclei in overlay (D, right panel). 3 µm optical projections. (E) Optical projections of whole brain-ventral nerve cord complexes from late 3rd instar larvae of the indicated genotype, approximately 130 hr AED, all matched in scale. CD8GFP (green) expression in all repo-Gal4-expressing glia reveals structure of the entire CNS. 200 µm scale bar. (F–H): Stg expression (red) in wild-type (F), repo>dEGFRλ;dp110CAAX (G), and repo>pntdsRNA;dEGFRλ;dp110CAAX (H) brains. Stg expression in glia is visible as purple nuclei in overlay (right panels). repo>pntdsRNA;dEGFRλ;dp110CAAX glia (H) show reduced Stg compared to repo>dEGFRλ;dp110CAAX glia (G, arrows indicate some Stg-expressing glia). Intense Stg expression (red only) in neuroblasts (‘NB’) and ganglion mother cell neuronal progenitors (‘NP’) is visible in all panels. Stg is visualized with the stgCB03726 protein trap, a viable stg allele that contains a GFP-expressing exon [68] and reflects the observed patterns of endogenous stg expression (data not shown). 5 µm optical projections. Genotypes: (A), (C), and (F) repo-Gal4 UAS-CD8GFP/+, (B), (D), and (G) UAS-dEGFRλ UAS-dp110CAAX/+; repo-Gal4 UAS-CD8GFP/+, (E) from left to right: repo-Gal4, UAS-CD8GFP/+, UAS-dEGFRλ UAS-dp110CAAX/+; repo-Gal4 UAS-CD8GFP/+, UAS-dEGFRλ UAS-dp110CAAX/+; repo-Gal4 UAS-CD8GFP/UAS-pntdsRNA, UAS-dEGFRλ UAS-dp110CAAX/+; repo-Gal4 UAS-CD8GFP/stg01235, UAS-dEGFRλ UAS-dp110CAAX/+; UAS-stg/+; repo-Gal4 UAS-CD8GFP/+, (F) stgCB03726/repo-Gal4 UAS-CD8GFP, (G) UAS-dEGFRλ UAS-dp110CAAX/+; stgCB03726/repo-Gal4 UAS-CD8GFP, (H) UAS-dEGFRλ UAS-dp110CAAX/+; stgCB03726 UAS-pntdsRNA/repo-Gal4 UAS-CD8GFP.