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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cancer Res. 2009 Jan 20;69(3):810–818. doi: 10.1158/0008-5472.CAN-08-2473

Figure 4.

Figure 4

CDK6 is required for Notch-dependent differentiation. A, Flow cytometry of WT and KO cells stained with CD4 and CD8. c-Kit+Lin- bone marrow cells were cocultured on OP9-DL1 stromal cells and analyzed by FACS at various time points as indicated. B, Flow cytometry of WT and KO cells stained with the ‘cocktail’ of lineage-specific antibodies and anti-CD44 and anti-CD25. Lineage-positive cells were electronically ‘gated out’ and CD44-versus-CD25 profiles of the lineage-negative compartments are presented. Numbers in quadrants indicate the percentage of cells in each subset stained with CD44 and CD25. C, Comparisons of flow cytometric analysis of CD25 expression in DN thymocytes from WT and KO cells (Figure 4B) collected from OP9-DL1 cocultures.