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. Author manuscript; available in PMC: 2009 Nov 7.
Published in final edited form as: Mol Cell. 2008 Nov 7;32(3):325–336. doi: 10.1016/j.molcel.2008.09.024

Figure 4. Two flap endonuclease activities in ME.

Figure 4

A. Flap endonuclease activities in ME. 1 µg non-depleted ME or a ME depleted of either hDNA2 or hFEN1 were incubated with 1 pmol 5’ end 32P labeled flap DNA substrates (upper panel). 100 ng hDNA2, or 10 ng hFEN1 were added to the reactions as specified. Reactions were carried out at 37°C for 5, 10, 30, and 60 min. B. hDNA2 and hFEN1 synergistically cleave flap substrates. 0, 2, 10, and 50 ng hDNA2 were incubated with 1 pmol 3’ end 32P labeled flap DNA substrates in the presence of 1 ng BSA or hFEN1. Reactions were carried out at 37°C for 60 min. The cleavage products were analyzed with 15% denaturing PAGE in both A and B.