Table 3.
Release of Standard Virus by wtDIP- and rDIP-Infected Cells
| Virus/DIP Preparation | Virus in Supernatant log pfu/mL±SD |
|---|---|
| Standard EHV-1 | 2.9±0.05 |
| Wt DIP | 6.1±0.10 |
| Empty vector | 2.2±0.04 |
| rDIP1 | 3.7±0.06 |
| rDIP2 | 2.7±0.09 |
| rDIP3 | 5.2±0.02 |
Persistent infection mediated by rDIP set up as described in the Materials and Methods. Monolayers of RK-13 cells were infected with the supernatant from cells infected with the standard virus, wild-type DIP generated from repeated high multiplicity infection with the standard virus (DI-172; 172nd high multiplicity passage) or recombinant DIP preparations (rDIP1, rDIP2, rDIP3; see Table 1 for genotypes). Plaques were quantitated after 24 h. Empty vector = cells transfected with pSV-β-galactosidase containing no DIP gene sequences (parent vector).