Figure 1. CD56^Dim and CD56^Bright subset determination.

A representative dot plot showing markers for CD56⁺ NK cell subsets (A). Following selection of CD3⁻ CD8⁻ cells, CD56^Dim and CD56^Bright cells can be identified by density of CD56 staining. A representative example of an ASD subject is shown for determination of frequency of IFNγ positive CD56^Bright cells (B) and frequency of CD56^Dim cells positive for perforin (C) and granzyme B (D). The shaded line denotes isotype control. The percentage of positive cells is denoted above each histogram.