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. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Mol Ther. 2003 Mar;7(3):419–425. doi: 10.1016/s1525-0016(03)00012-1

FIG. 2.

FIG. 2

Production of chimeric vectors. AAV1 and AAV2 chimeric vectors were generated by cotransfection of pAAV.F.IX, pAd, and AAV helper into 3 × 108 293 cells. The ratios of AAV1 and AAV2 helper are 10:0, 9:1, 1:1, 1:9, and 0:10. The vectors were purified by CsCl gradient. Vectors at the density of 1.38 -1.42 g/ml were collected. The titer was determined by quantitative PCR. The experiment was repeated three times and similar results were obtained. The y axis shows the total amount of vectors isolated in genomes. The standard deviation is identified.