FIGURE 10.

Changes in Dpo4 catalytic properties as a function of bulk of N²-alkyl G. A, k_cat/K_m values (Table 3) for dCTP (▪), dATP (•), dGTP (▴), and dTTP (□) incorporation opposite various N²-G adducts by Dpo4 as a function of molecular volume of N²-alkyl G adducts. The volumes of the adducts were calculated according to Connolly (81). B, k_cat/K_m values (Table 4) for next-base extension (with dGTP) from matched G (or N²-alkyl G adduct):C template·primer termini (▪) or mismatched G (or N²-alkyl G adducts):T template·primer termini (•) by Dpo4. C, k_pol/K_d values (Fig. 5) for dCTP incorporation by Dpo4 as a function of molecular volume of N²-alkyl G adducts.