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. 2009 Feb 6;284(6):3563–3576. doi: 10.1074/jbc.M807778200

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Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Estimation of k_pol and K_d_,dCTP for Dpo4 by pre-steady-state burst rate dependence on dCTP concentration. Dpo4 (200 nm) was incubated with 100 nm 24-mer/36-mer primer·template complexes (³²P-labeled) in a rapid quench-flow instrument and mixed with varying dCTP concentrations (2–300 μm) to initiate reactions. Plots of burst rates (k_obs, fit by Equation 2) versus [dCTP] were fit to a hyperbolic equation (Equation 3). A, G (unmodified); B, N²-MeG; C, N²-EtG; D, N²-IbG; E, N²-BzG; F, N²-NaphG; and G, N²-AnthG. The calculated k_pol and K_d values are shown in each panel of the figure.