TABLE 3.

Steady-state kinetic parameters for one-base incorporation opposite G and N²-alkyl G adducts by Dpo4

The extent of conversion of primer to the product was kept <20% by adjustment of the enzyme concentration and reaction time.

Template	dNTP	Km,dNTP	kcat × 103	kcat/Km × 105	Efficiency relative to dCTP:G	Misincorporation frequency
		μm	s-1	μm-1 s-1
G	C	1.2 ± 0.3	960 ± 50	8.2 × 104	1
	A	490 ± 60	5.7 ± 0.2	1.2	68,000-fold less	1.4 × 10-5
	G	190 ± 20	6.3 ± 0.1	3.3	25,000-fold less	4.1 × 10-5
	T	780 ± 140	34 ± 2	4.4	19,000-fold less	5.4 × 10-5
N2-MeG	C	1.9 ± 0.5	500 ± 21	2.7 × 104	3-fold less
	A	650 ± 70	7.6 ± 0.3	1.2	68,000-fold less	0.44 × 10-4
	G	380 ± 80	23 ± 2	6.1	13,000-fold less	2.3 × 10-4
	T	320 ± 90	40 ± 5	12	6,800-fold less	4.7 × 10-4
N2-EtG	C	7.4 ± 1.0	83 ± 3	1100	75-fold less
	A	360 ± 60	3.4 ± 0.2	0.93	88,000-fold less	0.82 × 10-3
	G	210 ± 30	5.5 ± 0.3	2.6	32,000-fold less	2.4 × 10-3
	T	78 ± 18	2.6 ± 0.1	3.4	24,000-fold less	3.0 × 10-3
N2-IbG	C	16 ± 3	100 ± 5	650	126-fold less
	A	280 ± 50	3.0 ± 0.2	1.1	75,000-fold less	1.6 × 10-3
	G	170 ± 10	8.8 ± 0.2	5.2	16,000-fold less	8.0 × 10-3
	T	140 ± 10	1.6 ± 0.0	1.2	68,000-fold less	1.9 × 10-3
N2-BzG	C	21 ± 4	270 ± 20	1300	63-fold less
	A	190 ± 30	2.1 ± 0.1	1.1	75,000-fold less	0.89 × 10-3
	G	120 ± 20	2.2 ± 0.1	1.8	46,000-fold less	1.4 × 10-3
	T	320 ± 53	4.4 ± 0.2	1.4	59,000-fold less	1.1 × 10-3
N2-NaphG	C	5.2 ± 1.0	110 ± 3	2200	37-fold less
	A	320 ± 60	1.0 ± 0.1	0.33	248,000-fold less	1.5 × 10-4
	G	180 ± 20	3.8 ± 0.1	2.2	37,000-fold less	9.8 × 10-4
	T	120 ± 20	1.5 ± 0.1	1.3	63,000-fold less	5.6 × 10-4
N2-AnthG	C	1.3 ± 0.15	43 ± 1	3200	26-fold less
	A	360 ± 450	0.78 ± 0.03	0.21	390,000-fold less	0.67 × 10-4
	G	870 ± 140	6.5 ± 0.5	0.75	109,000-fold less	2.3 × 10-4
	T	100 ± 10	2.3 ± 0.05	2.3	36,000-fold less	7.1 × 10-4