Skip to main content
. 2009 Feb 6;284(6):3563–3576. doi: 10.1074/jbc.M807778200

TABLE 5.

Products of extension of N2-alkylG template·primer complexes by Dpo4

Reaction conditions: 2.5 μm Dpo4, 5 μm 24/36 oligonucleotide complex, 1 mm each dNTP, 5 mm Mg2+, 4 h. LC-MS: tR 2.90–2.96 min; charge: –4; area: m/z value ± 0.2. The position of incorporation is indicated in italics.

5′-GCCUCGAGUCAGCCGUAGACGU AG
3′-CGGAGCTCGGTCGGCG TCT GCG TCG CTC CTG CGG CT
Unmodified G AGC GAG GAC GCC GA 17%
AGC GAG GAC GCC GAC 24%
AGC GAG GAC GCC GAA 59%
N2-MeG AGC GAG GAC GCC GA 27%
AGC GAG GAC GCC GAC 23%
AGC GAG GAC GCC GAA 50%
N2-EtG AGC GAG GAC GCC GA 93%
AGC GAG GAC GCC GAA 7%
N2-IbG AGC GAG GAC GCC GA 45%
AGC GAG GAC GCC GAC 11%
AGC GAG GAC GCC GAA 44%
N2-BzG AGC GAG GAC GCC GA 35%
AGC GAG GAC GCC GAC 20%
AGC GAG GAC GCC GAA 45%
N2-NaphG AGC GAG GAC GCC GA 96%
AGC GAG GAC GCC GAA 4%
N2-AnthG AGC GAG GAC GCC GA 56%
AGC GAG GAC GCC GAC 7%
AGC GAG GAC GCC GAA 16%
AGA GAG GAC GCC GA 21%