FIG. 7.

BMP2 stimulates chicken upper sternal chondrocytes to express RANKL. Lower sternal chondrocytes (A) and upper chondrocytes (B) from 15-day-old chick embryos were isolated and plated in 6-well plates for 7 days. The cells were treated with BMP2 (100 ng/ml) for various times. The expression levels of RANKL, Col II, Col X, Runx2, and GAPDH mRNA were determined by real-time RT-PCR. The fold induction of levels of individual genes was calculated as described in Fig. 1. ^a p < 0.05 vs. control value at the same time-point.