Table 1.

Sequences of primers used in the real-time PCR

Genes	Sequences of primers	GenBank accession number	Target sites on genes	Product sizes (bp)
Mouse
RANKL	F: 5’ CCAAGATCTCTAACATGACG 3’	BC125603	635–774	140
	R: 5’ CACCATCAGCTGAAGATAGT 3’
OPG	F: 5’ CAGAGCGAAACACAGTTTG 3’	U94331	697–497	201
	R: 5’ CACACAGGGTGACATCTATTC 3’
Col2	F: 5’ GATGACATTATCTGTGAAG 3’	NM_031163	345–494	150
	R: 5’ ATCTCTGATATCTCCAGG 3’
ColX	F: 5’ CTTTGTGTGCCTTTCAATCG 3’	NM_009925	3540–3677	138
	R: 5’ GTGAGGTACAGCCTACCAGTT 3’
OC	F: 5’ CTTGGTGCACACCTAGCAGA 3’	L24429	25–176	152
	R: 5’ ACCTTATTGCCCTCCTGCTT 3’
β-actin	F: 5’ ACCCAGATCATGTTTGAGAC 3’	X03765	280–503	224
	R: 5’ GTCAGGATCTTCATGAGGTAGT 3’
Chicken
RANKL	F: 5’ ACACGCCCTTTGAAAATCAG 3’	XM425625	305–500	196
	R: 5’ GCAAAAGGTTGCTTCTCTGG 3’
Runx2	F: 5’ ACTTTGACAATAACTGTCCT 3’	AF445419	511–702	192
	R: 5’ GACCCCTACTCTCATACTGG 3’
Col2	F: 5’ AGAAAGGAATCCAGCCCAAT 3’	NM_204426	4384–4621	238
	R: 5’ ACACCTGCCAGATTGATTCC 3’
ColX	F: 5’ ACATGCATTTACAAATATCGTTAC 3’	J04194	1340–1500	161
	R: 5’ AAAATAGTAGACGTTACCTTGACTC 3’
GAPDH	F: 5’ TATGATGATATCAAGAGGGTAGT 3’	K01458	813–1011	199
	R: 5’ TGTATCCAAACTCATTGTCATAC 3’

F, forward primer; R, reverse primer.