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. 2009 Feb 5;106(8):2812–2817. doi: 10.1073/pnas.0809579106

Fig. 4.

Fig. 4.

Molecular characterization of “engineered” MBs in mice after enforced expression of the miR-17∼92 cluster in GNPs from P6 Ink4c−/−; Ptch1+/− mice. Enforced expression of miR-17∼92 in P6 GNPs from Ink4c−/−; Ptch1+/− mice induced MBs in recipient mice. QRT-PCR analysis was performed in (A) and (B) using RNA extracted from purified GNPs from P6 mouse cerebella, 1-month (1M) whole mouse cerebellum, and GNP-like tumor cells from MBs engineered and spontaneously arising in Ink4c−/−; Ptch1+/− mice (MB 65939) (the numbers indicate the recipient mice). (A) miR-17∼92 expression in induced tumors was confirmed by analysis of selected individual microRNAs from the cluster (miR-19a, miR-92). (B) Induced tumors express high levels of Math1 and Gli1 mRNA. (C) GNP-like tumor cells overexpressing the miR-17∼92 cluster remain sensitive to cyclopamine: tumor cells from 2 independently derived MBs (tumor nos. 64199 and 64389) treated or not with cyclopamine were measured for proliferation by BrdU incorporation (Left panel) and for expression of Gli1 and Math1 RNA expression by QRT-PCR (Right panel).