Fig. 2.

FAC phosphorylation and retention require DCK expression. (A) Western blot of cell lines probed with anti-DCK and anti-actin antibodies. (B) IC₅₀ dFdC curves of the L1210 cell line panel. DCK-expressing cells display greater sensitivity to dFdC than DCK-deficient cells. Data are fitted as log (inhibitor) vs. response. (C) In vitro kinase assay with the L1210 cell lines with 1 μCi of [¹⁸F]FAC (Left) and [³H]dFdC (Right) as a substrate (1 μg of protein per reaction). (D and E) In vitro uptake assays with 1 μCi of ³H-labeled probes in the L1210 cell lines; 2-DG and FLT were negative controls.