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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Heart Rhythm. 2008 Sep 6;5(12):1715–1723. doi: 10.1016/j.hrthm.2008.09.009

Figure 6.

Figure 6

Panel A: Triton X-dependent segregation of DP in NRVM’s expressing PKP2 mutants R79x or 179fs. Fractions: T (total), I (insoluble) or S (soluble). Panel B: Similar experiment; blot probed for Cx43. In average of three experiments, ratio of low/high mobility band density was 0.96, 0.50 and 0.96 for samples obtained from T, S or I fractions of PKP2-FLAG-expressing cells. Numbers were similar for 179fs-expressing cells (0.97, 0.56 and 1.34) but lower in the T and I fractions of cells expressing R79x (0.51, 0.53 and 0.7). Cx43 band density in samples labeled “T” obtained from cells expressing R79x-FLAG was 37%, when compared to cells expressing wtPKP2-FLAG (band density adjusted for the corresponding actin control). ß-actin was used as loading control. Panel C: Dye transfer between NRVM cell pairs. Plot of averaged data (+/- SEM). Fluorescence intensity in each cell (relative to maximum in cell 1) was measured as a function of time after patch break. Graph shows rise in dye intensity in both control and R79X donor cells (■,●) and recipient cells (□,○) as a function of time.