Table 1.
Parasite Strain | % of infected cell | Amastigotes/100 cells | ||||
Medium | CK18 RNAi a | CTR RNAi a | Medium | CK18 RNAi a | CTR RNAi a | |
Y | 32.0 ± 4.95 | 30.0 ± 3.53 | 28.0 ± 2.83 | 578 ± 11.36 | 142 ± 20.66b | 497 ± 16.04 |
CL-Brener | 33.0 ± 2.0 | 35.0 ± 4.16 | 33.0 ± 4.04 | 411 ± 14.57 | 176 ± 18.55b | 406 ± 16.92 |
a HeLa cells were transiently transfected with CK18 or control RNAi 24 h earlier as described in Methods section. These cells were infected with culture trypomastigotes of Y or CL-Brener strain using the parasite per cell ratio of 10:1 or 5:1 respectively. The number of intracellular amastigotes was estimated 48 h post-infection and the results are expressed as average of triplicates ± SD. This experiment was performed 3 times with similar results.
b The number of amastigotes in cultures containing medium or RNAi control are lower than the number of amastigotes in cultures containing Medium with control RNAi (P < 0.01 in all cases, One Way Anova).