. 2008 Dec 17;9:68. doi: 10.1186/1471-2121-9-68

Table 1.

RNAi – medited knockdown of CK18 inhibits intracellular multiplication of amastigotes of Y or CL-Brener strains

Parasite Strain	% of infected cell			Amastigotes/100 cells
	Medium	CK18 RNAi ^a	CTR RNAi ^a	Medium	CK18 RNAi ^a	CTR RNAi ^a
Y	32.0 ± 4.95	30.0 ± 3.53	28.0 ± 2.83	578 ± 11.36	142 ± 20.66^b	497 ± 16.04
CL-Brener	33.0 ± 2.0	35.0 ± 4.16	33.0 ± 4.04	411 ± 14.57	176 ± 18.55^b	406 ± 16.92

^aHeLa cells were transiently transfected with CK18 or control RNAi 24 h earlier as described in Methods section. These cells were infected with culture trypomastigotes of Y or CL-Brener strain using the parasite per cell ratio of 10:1 or 5:1 respectively. The number of intracellular amastigotes was estimated 48 h post-infection and the results are expressed as average of triplicates ± SD. This experiment was performed 3 times with similar results.

^bThe number of amastigotes in cultures containing medium or RNAi control are lower than the number of amastigotes in cultures containing Medium with control RNAi (P < 0.01 in all cases, One Way Anova).