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. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Oncogene. 2008 Nov 17;28(5):651–661. doi: 10.1038/onc.2008.419

Figure 1.

Figure 1

HIC1 interacts with Brg1. (a) Extracts of HSF8 cells were analysed by immunoprecipitation (IP), PAGE and immunoblotting (IB) using the indicated antibodies. CE represents cell extract before IP. (b) Extracts from MCF7 cells (control) or MCF7 cells transfected with Flag-tagged HIC1 (Flag-HIC1) were analysed by PAGE and IB, using the indicated antibodies. (c) Cell extracts of MCF7 cells transfected with Flag-HIC1 shown in panel b were immunoprecipitated by the indicated antibodies, followed by PAGE and IB using the antibodies shown. (d) The 35S-labeled proteins indicated were incubated with GST or the indicated fusion proteins, followed by pull-down analysis using standard protocols. RL represents the 35S-labeled proteins without binding. GST, glutathione S-transferase; PAGE, polyacrylamide gel electrophoresis.