Figure 7.

HIC1 is required for serum deprivation-induced growth arrest. HSF8 cells were stably transfected with an empty vector, or control siRNA, or HIC1 siRNA. The cells were serum starved for 72 h and their cell cycle profiles were analysed by propidium iodide staining and flow cytometry. The experiments were independently repeated four times, yielding similar results. Profiles from one representative experiment are shown, with the percentages of cells in each phase of the cell cycle derived from the four independent experiments indicated. siRNA, small interfering RNA.