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. Author manuscript; available in PMC: 2009 Feb 5.
Published in final edited form as: Oncogene. 2008 Nov 17;28(5):651–661. doi: 10.1038/onc.2008.419

Figure 8.

Figure 8

Cell cycle dependency of HIC1 versus E2F promoter occupancy. (a) HSF8 cells were serum starved for 72 h (G0), and one portion was then treated with serum for 12 h (late G1). The E2F1 and HIC1 genes in these HSF8 cells were analysed for transcription factor occupancy by quantitative ChIP or double-ChIP assays, using the indicated antibodies. (b) The E2F1 and HIC1 genes in these HSF8 cells were analysed for activity by quantitation of transcripts. The experiments were repeated four times, which yielded comparable results, and the error bars shown represent the s.e.m. from all four assays. ChIP, chromatin immunoprecipitation.