Table 1.

HIC1 is required for growth suppression by serum starvation

Vector transfected	Number of colonies
	Experiment 1	Experiment 2	Experiment 3
Empty vector, 0.1% FBS	5	3	1
Empty vector, 10% FBS	409	434	411
Control siRNA, 0.1% FBS	3	1	4
Control siRNA, 10% FBS	435	446	415
HIC1 siRNA, 0.1% FBS	456	398	408
HIC1 siRNA, 10% FBS	468	446	461

Abbreviation: FBS, fetal bovine serum.

Approximately 10 000 HSF8 cells were transfected with 2 μg of the indicated vectors. Seventy-two hours after transfection, serum (FBS) in the medium was decreased to 0.1% for serum starvation or the culture was continued with normal serum concentrations (10% FBS), as indicated. The cells were selected in 100 μg of G418 per ml for 14 days. Colonies with 20 or more cells were counted.