Figure 2. The influence of 5′UTRs and 3′UTRs on transcript stability and translational efficiency.

The 5′-UTRs and the 3′-UTRs of the hlr gene and the hp gene (Figure 1A–B) were fused to the dhfr reporter gene alone or simultaniously. (A) The transcript fusions are shown schematically. The DHFR enzymatic activities, the dhfr transcript levels and the translational efficiencies of exponential and stationary growth phases are tabulated. Three biological replicates were performed and average values with standard deviations (in parenthesis) were calculated. (B) The translational efficiencies after normalization to the control transcript without UTRs (No. 1) are shown.