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. 2009 Feb 9;4(2):e4417. doi: 10.1371/journal.pone.0004417

Figure 11. Protein profiles of NLP and NB cultured from serum.

Figure 11

(A) Protein profiles of (A) NB cultured from HS and DMEM, as in Fig. 3; (B) NB strain DSM 5819 subcultured in serum-free DMEM for 2 days; (C) FBS NLP prepared as in Fig. 3 in the presence of 5% FBS; (D) HS NLP prepared as in Fig. 3 in the presence of 5% HS. (E) NB as in (A) after three 2-day serial passages through serum-free DMEM. (F) Lane 1: “Nanons” after two 2-day passages in serum-free DMEM. Lane 2: “Nanons” after two 2-day passages in DMEM containing 10% FBS. Gels were stained with Coomassie blue. The protein samples loaded onto each lane and their preparation are described in the Materials and Methods .