Figure 14. Protein identification in NLP prepared from FBS and HS.

Protein profiles correspond to Figs. 11C and D, reproduced here to illustrate the protein identification procedure used as well as the results obtained. NLP were prepared from DMEM containing 5% FBS or 5% HS that had been precipitated with 10 mM each of CaCl₂, Na₂CO₃, and NaH₂PO₄. Following SDS-PAGE and Coomassie blue staining, the bands were excised (white dots) and submitted to identification by MALDI-TOF mass fingerprint analysis. The proteins identified are given along with their positions. Abbreviations used: BSA, bovine serum albumin; BSF, bovine serum fetuin-A; b Apo-A1, bovine apolipoprotein A1; HSA, human serum albumin; HSF, human serum fetuin-A; and h Apo-A1, human apolipoprotein A1. Other minor protein bands include a bovine 95 kDa band (▪) which was identified as bovine serotransferrin precursor (2 out of 2 trials, or 2/2), and other minor human bands at 165 kDa (•), 130 kDa (♦), 95 kDa (◃) identified as human complement C3 (2/2), human antithrombin (2/2), and human complement C3 (2/2), respectively.