Figure 15. Proteins identified in NLP prepared from various body fluids.

NLP were prepared from body fluids in the presence of CaCl₂, Na₂CO₃, and NaH₂PO₄ added to final concentrations of 9 mM each for saliva, 7 mM for urine, and 8 mM for the other body fluids. Following SDS-PAGE, the NLP proteins identified by MALDI-TOF mass fingerprint analysis are shown next to each major band. Other minor bands in the 50–65 kDa range marked by the symbol (◃) include (B) IgG chain C region (obtained 1 out of 1 trial, or 1/1); (C) IgG chain C region (1/1); (D) HSA (1/3), IgG chain C region (1/3), and α-anti-trypsin (1/3); (E) HSA (2/2); and (F) vitamin-D binding protein (1/3), IgG chain C region (1/3), and Apo-A4 (1/3).