Skip to main content
. 2009 Feb 9;4(2):e4417. doi: 10.1371/journal.pone.0004417

Figure 18. NB-specific antibodies cross-react with albumin and fetuin-A.

Figure 18

Western blots on various bovine and human protein samples (bottom of each blot) were done using the indicated antibodies (to the right of each blot). Antigens used include FBS NLP and HS NLP obtained as in Fig. 14; purified BSA, BSF, HSF, and human recombinant fetuin-A (HRF); and whole FBS and HS. Antibodies used include a polyclonal antibody directed against BSF (p-α-BSF); a monoclonal antibody directed against BSA (m-α-BSA); a polyclonal antibody directed against HSF (p-α-HSF); and the monoclonal antibodies 8D10, NB 5/3, and NB G1B8 directed against bovine NB antigens (m-α-NB 8D10, m-α-NB 5/3, and m-α-NB G1B8, respectively). All gels were run under denaturing and reducing conditions except for (L and M), which were electrophoresed under non-reducing conditions.